Therefore, to further explore the regulatory function of TOLLIP in NSCLC, we transfected TOLLIP overexpression plasmids into A549 and HCC827 cells and successfully overexpressed TOLLIP (increased 2.1-fold (A549) and 1.9-fold (HCC827)) (Fig. 5A). The gene discussed is TOLLIP; the disease is non-small cell lung carcinoma.