To demonstrate that FASN, PLAUR, BCL2L1, HMOX1 and RHOB are powerful and promising diagnostic biomarkers of CTEPH for Anoikis, we conducted validation experiments utilizing dataset GSE188938, qRT-PCR, and Western blot to assess the expression of Anoikis-related genes in the CTEPH and control groups. This evidence concerns the gene RHOB and chronic thromboembolic pulmonary hypertension.