Given that IRG1 was mainly expressed in neutrophils and macrophages during viral infection, and CD45- non-immune cells exhibited minimal expressed IRG1 (Fig. 6d), we next investigated whether the IRG1-itaconate axis regulates viral infection in vivo through immune cells by generating Irg1 immune lineage-deficient mice (Fig. 7a). This evidence concerns the gene ACOD1 and viral infectious disease.