To determine whether the NLRP3 inflammasome is activated after PEDV infection, we detected its expression and found that the NLRP3 inflammasome mRNA expression increased in a time- and dose-dependent manner following the infection of IPEC-J2 cells with PEDV (Figure 3a,b), demonstrating that the PEDV infection of IPEC-J2 cells promoted NLRP3 mRNA expression. The gene discussed is NLRP3; the disease is infection.