In this article, we present a protocol for lentiviral transduction of EOC organoids, with a particular focus on the knockdown of the metastasis-associated in colon cancer 1 (MACC1) gene in representative high-grade serous ovarian cancer (HGSOC) lines, the most common and most aggressive form of EOC. This evidence concerns the gene MACC1 and ovarian serous adenocarcinoma.