Meanwhile, based on the degree of tumor immune cell infiltration and different m5C regulators, it has been discovered that NSUN3 is closely related to CD8+ T cells, while NSUN4 is closely related to neutrophils, confirming that m5C can regulate the tumor immune microenvironment to predict the prognosis of lung squamous cell carcinoma (67). The gene discussed is NSUN3; the disease is squamous cell lung carcinoma.