EVs secreted by NK cells carry signature NK markers and cytotoxic proteins, such as granzyme A and B, granulysin, and perforin, which mediate antitumor effects against solid tumors, such as neuroblastoma, breast cancer, and non-small cell lung cancer (NSCLC) (Lugini et al. 2012; Jong et al. 2017; Wu et al. 2019). The gene discussed is PRF1; the disease is non-small cell lung carcinoma.