In U251 astrocytoma cells, sevoflurane treatment caused a reduction in cell viability, upregulation of inflammatory factors, a rise in ROS concentrations and changes in the expression of SLC7A11 and related ferroptosis regulatory factors (GPX4, ACSL4, FPN1, TFR1, FTH1, NRF2 and HO‐1), further supporting the hypothesis that sevoflurane induces astrocyte ferroptosis through the regulation of SLC7A11. This evidence concerns the gene GPX4 and astrocytoma (excluding glioblastoma).