Therefore, to test the ability of this approach to diagnose newly diagnosed cases of presumed germline RB, we carried out genomic DNA extraction, library preparation, and sequencing over 12 hours, targeting a panel (Oncoplex) (35) of 377 cancer-causing genes (to identify any potential non-RB1 causative variants) and chromosome 13 (to capture any large SVs affecting RB1) for 12 hours. The gene discussed is RB1; the disease is cancer.