Furthermore, for most HPV genotypes, mean Allplex HPV28 Ct-values of infections detected by both Allplex HPV28 and GP5+/6+ RLB AF were significantly lower than infections detected only by Allplex HPV28, suggesting that GP5+/6+ RLB AF negativity was, at least in part, related to viral load. This evidence concerns the gene GP5 and infection.