From our study we conclude that Juzentaihoto-induced polarization of tumor-associated macrophages into the M1 phenotype not only induces anti-tumor immune-cell activity and cytokine release (such as TNF-α, IL6), it also ameliorates Gemcitabine efficacy in view of DNA-analogue as well as partial antitumor antigen. The gene discussed is TNF; the disease is neoplasm.