Forty male Wistar rats, aged 16 weeks, were divided into four groups with ten rats each. T1DM was induced using 40 mg/kg Streptozocin, administered 2 months after implant placement. On day 2 post-induction, all rats exhibited signs of diabetes. They serve as a model for understanding the effects of insulin treatment and diabetes management. The gene discussed is INS; the disease is diabetes mellitus.