In line with TNF-pretreatment of pediatric sarcoma cell lines not promoting anti-tumor activity of unspecific PBMCs (Figure 5), TNF-pretreatment of A673 and TC32 did not enhance IFNγ release of unspecific CD8+ T cells (Figures 7A, B, bottom panels), further indicating that TNF does not promote MHC-I independent cytotoxicity. Here, CD8A is linked to sarcoma.