We used a panel of EwS, RMS and OS pediatric sarcoma cell lines to test six cytokines, used for monocyte differentiation and DC maturation (IL-4, GM-CSF, IL-1β, IL-6, TNF and PGE2), for their ability to induce cancer cell immunogenicity and sensitivity to tumor antigen-specific T cells. This evidence concerns the gene CSF2 and cancer.