To test if the surface expression of immunogenic markers can be enhanced, pediatric sarcoma cell lines were incubated for 96 h with individual cytokines commonly used together for the differentiation and maturation of monocytic-derived dendritic cells (DC), including IL-4, GM-CSF, IL-1β, IL-6, TNF and PGE2 (13, 16). This evidence concerns the gene TNF and sarcoma.