Notably, SJSA-1 and U2OS cells showed PD-L1 expression (Figure 1, Supplementary Figure 2A), demonstrating a mixed expression of immunogenic and immunosuppressive markers among pediatric sarcomas; which is in accordance with partial PD-L1 expression in osteosarcomas (5, 23, 24). The gene discussed is CD274; the disease is osteosarcoma.