Quantitative analysis of the TEER demonstrated that tango2-MOspE3 induces a locomotor phenotype, as shown by significantly decreased distance (Figure 2D), which is consistent with recent observations in CRISPR KO tango2 models.15,16 To validate the specificity of this locomotor phenotype, we exposed 30 hpf zebrafish to atorvastatin (ATV, Lipitor), usually employed for the treatment of hypercholesterolemia, and proceeded to TEER analysis at 50 hpf. Here, TANGO2 is linked to Hypercholesterolemia.