Glycation is also a possible cause for SOD1 aggregation in Amyotrophic Lateral Sclerosis as shown in yeast expressing hSOD1 and lacking glyoxalase 1 for MGO detoxification.215 The succinylation of K122 has no effect on SOD1 activity, but suppresses the ability of SOD1 to inhibit mitochondrial metabolism as proven by K122E mutation in cells.216 Multiple acylations ameliorated SOD1 seeding capability and thereby prevented further protein aggregate formation.217. Here, SOD1 is linked to amyotrophic lateral sclerosis.