Two genes related to cellular metabolism (dipeptidase 1 (<i>DPEP1</i>) and sphingosine kinase 1 (<i>SPHK1</i>)) were found to be related to the PFOA-promoted formation of ulcerative colitis using our self-developed Metabolic Gene and Pathway Query software and Comparative Toxicogenomics Database (CTD). This evidence concerns the gene DPEP1 and ulcerative colitis.