To further explore the mechanism by which B68 affects the expression of PD‐L1, we measured the expression levels of PD‐L1 mRNA in colorectal cancer cells after treatment with B68 or treated tumor cells in combination with the protein synthesis inhibitor cycloheximide (CHX) to assess the changes in PD‐L1 protein expression. The gene discussed is CD274; the disease is colorectal cancer.