MAP1LC3A and infection: Autophagy is a dynamic process that involves the formation of autophagosomes, their fusion with lysosomes, and the degradation of autolysosomes.[48, 53] By monitoring intracellular fluorescence changes following infection with mRFP‐GFP‐LC3, we can observe the cellular autophagic flux more vividly and clearly, which serves as a reliable indicator to accurately assess autophagic activity.[54] Lysosomes are an acidic environment.