One-step nucleic acid amplification (OSNA) is a rapid intraoperative molecular testing technique to quantitatively assess tumor burden in resected lymph nodes of lung cancer patients by quantitatively measuring keratin 19 (CK19) mRNA, which provides high diagnostic accuracy and speed for detection of LNM, and can be applied to intraoperative decision-making for personalized lung cancer surgery (70, 71). This evidence concerns the gene KRT19 and neoplasm.