MAPT and frontotemporal dementia: To design the single-guide RNA (sgRNAs) to introduce FTD-associated pathogenic mutations into the MAPT KI mice using BE3, we searched for the targetable Cs in the fourth to eighth position of sgRNAs with -NGG (BE) or -NGA (VQR-BE) PAM sequences and found that MAPT-P301 (CCG) and MAPT-Int10+3 were suitable for the generation of mutant mice carrying MAPT-P301L, S (CTG or TCG) with or without the MAPT-Int10+3G>A mutation (Fig. 1a)1,3,30.