To further explore the roles of CXCR3 in spinal macrophage recruitment and pain in EAP mice, CXCR3 inhibitor AMG487 was administrated to EAP mice, and blockade of CXCR3 reduced the number of infiltrated spinal macrophages and pain responses, which demonstrated that the CXCL10/CXCR3 axis was involved in spinal macrophages recruitment and pain in chronic prostatitis. This evidence concerns the gene CXCL10 and prostatitis.