Because mast cell and NGF have important roles in pain in chronic prostatitis, cromolyn sodium and tanezumab (ip and intra‐prostatic injection) were simultaneously administrated to EAP mice, and the results showed that cromolyn sodium and tanezumab could reduce pain in EAP mice, and the spinal macrophages were also decreased, showing that targeting mast cell and NGF was warranted for chronic prostatitis, and more exploration should be conducted to demonstrate their combined efficacies in pain management. This evidence concerns the gene NGF and chronic prostatitis.