Previous studies found that the activated microglia and astrocytes in the spinal cord were associated with pain response in prostatitis [41, 42], and pain was relief and pain‐associated mediators including IL‐1β and BDNF were decreased after inhibition of the activated microglia [42], and spinal c‐fos expression was associated with central sensitisation in prostatitis model [43]. Here, FOS is linked to medical procedure.