To examine the specificity of the effect of Tat‐Nrf2 peptide on SENP6‐Nrf2 interaction, we investigated the effects of Tat‐Nrf2 on the SUMOylation of ANXA1, which is a confirmed substrate of SENP6, and we previously had demonstrated that SENP6 can bind to and mediated the deSUMOylation of ANXA1 after ischemic stroke.[20, 21] As presented in Figure S8 (Supporting Information), Tat‐Nrf2 had no impact on the binding of SENP6 with ANXA1 and the SUMOylation level of ANXA1 after OGD/R. This evidence concerns the gene ANXA1 and ischemic stroke.