In a liver metastasis model of colon cancer, the results showed that nuclear TRAF4 overexpression in TRAF4‐KO HCT116 cells resulted in the formation of more and larger metastatic nodules in livers compared with the TRAF4‐KO or cytoplasmic TRAF4 group (Figure 3J–L). This evidence concerns the gene TRAF4 and malignant colon neoplasm.