N‐glycosylation modification of CTSD appears to play distinct roles depending on the specific N‐glycosylation structures, species, or tissues involved.[19] Previous studies have demonstrated that the N‐glycosylation modification of CTSD is essential for its proper localization to lysosomal compartments.[19, 20] This finding is also corroborated by our observation that CTSD missing the N‐glycosylation modification at residue 263 or both 134/263 cannot co‐locate with endosomal/lysosomal markers in CRC. Here, CTSD is linked to colorectal carcinoma.