Detailed, IL‐10 has been reported to facilitate the formation of bacterial biofilm, thereby enabling S. aureus to escape from the host immune clearance.[101, 102, 103] IL35 could suppress the differentiation of CD4+T cells into Th17 cells, thereby dampening the immune response against infections.[104] TGF‐β suppressed the immune activities of Th1 and Th2 cell.[105] Our scRNA‐seq studies revealed a significant enrichment of genes involved in above cytokines in Treg from S. aureus PJI, meaning that the anti‐bacterial activity of host would be suppressed by the Treg. This evidence concerns the gene IL10 and infection.