Importantly, AAV–VDBP + CUMS group mice showed exacerbation of depression‐like behaviors, including significantly decreased SPT scores (Figure 2j) (p < 0.05), increased immobility times in the TST (p < 0.05) (Figure 2l) and in FST (p < 0.05) (Figure 2m), suggesting that the targeted overexpression of VDBP aggravates the CUMS‐induced depressive phenotypes, further revealing the importance of VDBP in the pathogenesis of depression. This evidence concerns the gene GC and major depressive disorder.