Compared with AAV–mCherry control mice, AAV–VDBP mice had shown abnormal behaviors, as indicated by decreased residence time in the central area of the OFT (Figure 2f) (p < 0.05), decreased sucrose preference test (SPT) scores (p < 0.05) (Figure 2e), and increased immobility times in the TST (Figure 2g) (p < 0.05) and FST (Figure 2h) (p < 0.001), suggesting that overexpression of MG‐derived VDBP in PrL region can induce depression‐like behaviors in mice. This evidence concerns the gene PRL and major depressive disorder.