Membrane binding sites have been identified in amino acids 150–172 and 379–402 of VDBP.[6] Interestingly, mice lacking VDBP do not show evidence of vitamin D deficiency unless placed on a vitamin‐D‐deficient diet.[34] The present study focused on the vitamin‐D‐independent role of VDBP which relied on megalin and downstream signaling pathways. This evidence concerns the gene GC and vitamin D deficiency.