In the multivariate Cox regression analysis of OS, baseline AFP ≥ 400 ng/ml and initial tumor shape irregularity were found to be significant independent prognostic factors for OS after adjusting for potential confounders (AFP ≥ 400 ng/ml, HR 1.73, 95% CI 1.01–2.96, P = 0.046; Initial tumor shape irregularity, HR 1.94, 95% CI 1.03–3.65, P = 0.041; Table 2). The gene discussed is AFP; the disease is neoplasm.