Meantime, overexpression of sirt1 significantly decreased the number of N. caninum tachyzoites per vacuole at 30 hpi (Fig. 6D) and 42 hpi (Fig. 6E), while the opposite effect on the infection burden of this parasite was detected for si-sirt1 at 30 hpi (Fig. 6D) and 42 hpi (Fig. 6E). Here, SIRT1 is linked to infection.