For radiosensitizing effects, HIF-1α-AuNPs + RT (6 Gy) and siRNA-AuNPs + RT showed greater tumor inhibition effects compared to the other group, nevertheless, HIF-1α-AuNPs + RT had the smallest tumor size, the highest level of tumor necrosis and cell nuclear breakdown and the highest level of γ-H2AX. The gene discussed is H2AX; the disease is neoplasm.