GNAI1 and urinary bladder cancer: Figure 7 shows that, unlike in T24 bladder cancer cells, YM, CTX, and PTX all inhibited NECA-induced Ca2+ increase although to a different extent. Thus, Gi, Gs and Gq are all involved in the A2BAR-mediated Ca2+ increase in MDA-MB-231 cells. Furthermore, CTX and PTX together produced an effect larger either alone (Fig. 7B). The selective A2BAR antagonist PSB603 (1 μM) completely blocked the effect of NECA (Fig. 7B).