Figure 7 shows that, unlike in T24 bladder cancer cells, YM, CTX, and PTX all inhibited NECA-induced Ca2+ increase although to a different extent. Thus, Gi, Gs and Gq are all involved in the A2BAR-mediated Ca2+ increase in MDA-MB-231 cells. Furthermore, CTX and PTX together produced an effect larger either alone (Fig. 7B). The selective A2BAR antagonist PSB603 (1 μM) completely blocked the effect of NECA (Fig. 7B). The gene discussed is GNAI1; the disease is urinary bladder carcinoma.