To investigate the mechanistic basis of the observed heterogeneous response, especially in the more aggressive β-M model, we employed the 10X Visium platform to perform unbiased spatial transcriptomics on an LNP-CTRL treated β-M HCC (“ β-M Control”), 2 LNP-CTNNB1 treated β-M HCC showing minimal/no response (“ β-M NR-1”; “ β-M NR-2”), and an LNP-CTNNB1 treated β-M HCC showing response (“ β-M R-1”). This evidence concerns the gene CTNNB1 and hepatocellular carcinoma.