To further interrogate tumor cell intrinsic biological effects that occurred at the 3-day timepoint, we administered LNP-CTRL or LNP-CTNNB1 at 5-weeks post-HDTVi to β-N model mice and performed single-cell RNA-sequencing (scRNA-seq) analysis on a hepatocyte-enriched single-cell population following whole liver perfusion. Here, CTRL is linked to neoplasm.