Although the antibody used in this study (Anti-hNSE 9601) has a higher affinity for both human NSE and recombinantNSE compared to the ECLIA (Anti-h NSE 18E5 and Anti-h NSE 84B10; sandwich),the developed intact top-down LC-MS assay was found to be less sensitivecompared to the ECLIA.44 The diagnosticperformances of the MS and the ECLIA in discriminating NSCLC and SCLCfrom benign cases were similar, although no significant conclusioncould be drawn due to the limited sample size. The gene discussed is ENO2; the disease is non-small cell lung carcinoma.