Furthermore, within these three types of cells with elevated expression of the gene module, we found specific interactions between the M1-high group of tumor cells and microglia, BMDMs cells, and stromal cells through various axis pathways (ANXA1-FPR1, TGFB1-(TGFBR1+TGFBR2), GRN-SORT1, GAS6-MERTK, ANGPTL4-(ITGA5+ITGB1), ANGPTL4-CDH11, and SPP1-(ITGAV+ITGB1)) (Fig 4G). This evidence concerns the gene SPP1 and neoplasm.