To confirm the relationship between BTF3 and PDCD2L, we first observed the expression level of PDCD2L in hepatocellular carcinoma cells knocking down and overexpressing BTF3, and the results showed that the expression of PDCD2L was in the same trend of change as that of BTF3 (Fig. 3D, E). The gene discussed is BTF3; the disease is hepatocellular carcinoma.