Considering previous research results, we hypothesized that TRAF6 may promote NF-κB-p65 nuclear translocation via suppressing IRF3, so we simultaneously performed nuclear-cytoplasmic separation and IF experiments in wild-type, shTRAF6, shTRAF6/shIRF3, and shIRF3 5-FU-resistant GC cells. Here, NFKB1 is linked to gastric cancer.