As expected, IRF3 knockdown rescued the reduction of NF-κB-p65 into the nuclear after TRAF6 knockdown (Fig. 5E, F), so our results show TRAF6 promotes the nuclear translocation of NF-κB-p65 via suppressing IRF3, thereby reducing 5-FU sensitivity in GC cells. The gene discussed is IRF3; the disease is gastric cancer.