Additionally, overexpression of TRAF6 in BGC-823-R and SGC-7901-R cells increased NF-κB-p65 nuclear translocation (Supplementary Fig. 3B), indicating high expression of TRAF6 promotes NF-κB-p65 nuclear translocation in 5-FU-resistant GC cells. The gene discussed is NFKB1; the disease is gastric cancer.