NEUROG2 and infection: Subsequently, Sendai viruses (SeV) carrying mouse transcription factors Lhx3, Ngn2, and Isl1 (SeV-Lhx3-Ngn2-Isl1), which play critical roles in LMN development, were transfected at a multiplicity of infection (MOI) of 5 (Goto et al., 2017).