Additionally, we observed a significant decrease in the frequency of multiple lymphoid populations in the cryoablated tumors, such as infiltrating NKT cells, T cells, other subpopulations of CD4+ T cells (Naïve, ICOS+, effector/effector memory), and subpopulations of CD8+ T cells (CD107a+ and effector/effector memory) (Figure 3A), as well as myeloid populations, such as CD8a+ XCR1+ (resident) DCs, Ly6Gint neutrophils, and M1-like macrophages (Figure 3B) compared to the resected baseline control tumor. This evidence concerns the gene CD4 and neoplasm.