Having validated the accuracy of HistoTME on CPTAC-NSCLC data, we next tested HistoTME on whole slide H&E images of 82 NSCLC patients from SUNY Upstate Medical University, which had serial immunohistochemistry (IHC) performed on surgical resection specimens for immune cell panel: T cells (CD3, CD4, DC8), B cells (CD20) and Macrophage (CD163) markers using adjacent serial sections (Supplementary Fig. 1). This evidence concerns the gene CD4 and non-small cell lung carcinoma.