Also, the effect of ageing on TRIM28 expression and its associations with both DLK1 and NOTCH3 should be studied profoundly, due to the previous association between TRIM28 and testicular degeneration and age-dependent infertility in mouse92 as well as our observed negative correlation between placental TRIM28 expression and maternal age. This evidence concerns the gene NOTCH3 and Testicular atrophy.