Through the CLRN1-AS1/miR-217/DKK1 axis, the ectopic expression of CLRN1-AS1 suppresses the cell proliferation and clonogenicity, increases caspase-3 activity, decreases tumor growth in vivo, and inhibits the Wnt/β-catenin signaling pathway. This evidence concerns the gene CLRN1 and neoplasm.