Recently, we reported the anti-osteoporotic activity of an ethyl acetate fraction (LEA) of shiitake mushroom (Lentinula edodes), which suppressed RANKL-induced osteoclastogenesis (Lee et al. 2020a, b); however, the precise molecular mechanisms underlying its preventative effects on osteoporosis are unknown. Here, TNFSF11 is linked to osteoporosis.