The objectives of the present study are as follows: (1) to establish the role of BEST4 in CRC growth both in vitro and in vivo; (2) to determine the underlying molecular mechanisms by which BEST4 interacts with HES4 and TWIST1, thereby inducing EMT; and (3) to investigate the correlation between BEST4 expression and prognosis of CRC. Here, HES4 is linked to colorectal carcinoma.