We also evaluated the inhibitory role of BEST4 in a model of CRC to the liver metastasis by intrasplenically injecting HCT-15 cell lines with BEST4 knockout (BEST4gRNA) or the wild-type control (gRNA) or the knockout with the rescue (BEST4-Rescued) into BALB/c nude mice. This evidence concerns the gene BEST4 and colorectal carcinoma.