To evaluate the effect of BEST4 on tumour growth, we constructed BEST4-expressing HCT116 and Caco2 CRC cell lines (Figure 1—figure supplement 1) and showed that BEST4 overexpression halved cell proliferating rates of their respective empty vector (EV) controls when they were monitored in live and analysed using InCucyte (Figure 1A and B). This evidence concerns the gene BEST4 and colorectal carcinoma.