Conversely, reducing HK2 expression promotes cytochrome C release into the cytoplasm, ultimately leading to apoptosis in tumor cells.39,40 The results in Figure 2e imply that Daurisoline increases the apoptosis of lung cancer through HK2-mediated cytochrome C, which was further validated by the observation that overexpressing HK2 reversed the effect of Daurisoline in HCC827 cells. The gene discussed is CYCS; the disease is lung cancer.