Through flow cytometric analysis (Figure 3A–C) and immunostaining (Figure 3D–G), we demonstrated that within 14 days postischemic stroke, the proinflammatory characteristics of microglia and macrophages gradually accumulated, as evidenced by the progressively elevated expression of IFN‐γ and TNF‐α (Figure 3B,D,E). This evidence concerns the gene TNF and Stroke.