Through flow cytometric analysis (Figure 3A–C) and immunostaining (Figure 3D–G), we demonstrated that within 14 days postischemic stroke, the proinflammatory characteristics of microglia and macrophages gradually accumulated, as evidenced by the progressively elevated expression of IFN‐γ and TNF‐α (Figure 3B,D,E). The gene discussed is TNF; the disease is stroke disorder.