Initially, the presence of Notch ligands or receptors in EVs was not directly detected, but Boelens et al. observed that vesicles released from stromal cells induced Notch3 activation in breast cancer cells[100], whereas Wang et al. reported that breast cancer cells release EVs promoting breast cancer cell drug resistance via Notch1 activation[101]. The gene discussed is NOTCH3; the disease is breast carcinoma.