Outcomes of 634 ICSI cycles from 634 couples, including 398 ICSI cycles with E-S from 398 couples and 236 ICSI cycles with T-S from 236 couples, demonstrated that T-S, low male LH levels, uterine anomalies, female infertility of multiple etiologies, and advanced maternal age were independently associated with adverse ICSI outcomes. Here, PLOD1 is linked to female infertility.