Multivariate logistic regression analysis suggested that factors, including the use of T-S (a-OR, 0.273; 95% CI, 0.156–0.468), infertility caused by uterine anomaly (a-OR, 0.101; 95% CI, 0.005–0.529), male LH levels (a-OR, 0.912; 95% CI, 0.837–0.990), and increased maternal age (a-OR, 0.873; 95% CI, 0.795–0.958) were independently associated with a low cumulative live birth rate (Fig. 3). The gene discussed is PLOD1; the disease is Infertility.