The results showed that in tumor tissues, the CD8/CD4 ratio was significantly higher in the NP/PTX/siCD47 and NP/PTX/siCD47/R848 treatment groups compared with the control group and the PTX single-agent nanoparticle group, and the increase in the secretion of granzyme B by the CD8+ T cells also reflected their highly efficient activation level, suggesting a potent activation of the adaptive immune system (Figures 6A–C; Supplementary Figure S6B), whereas the elevation of the percentage of B cells in the spleen also flanks the intrinsic immune system was activated (Supplementary Figure S6C). The gene discussed is CD8A; the disease is neoplasm.