In the present study, we found that tumor cells incubated with NPs and co-cultured with BMDCs and BMDMs that had been induced in vitro up to day 6 in a direct-contact manner significantly promoted the maturation and activation of BMDCs and BMDMs, resulting in elevated expression of IAIE, CD80 and CD86 and others on their surfaces. This evidence concerns the gene CD80 and neoplasm.