To further explore TSSC4-related signaling pathways in cancer cells, we performed Gene Set Enrichment Analysis (GSEA) for the GO pathways and KEGG pathways and identified signaling pathways related to the regulation of type-I interferon, NF-κB binding, focal adhesion, colorectal cancer, the Rap1 signaling pathway, and leukocyte transendothelial migration. The gene discussed is NFKB1; the disease is cancer.