Animal experimental research indicates that acupuncture stimulation of relevant acupoints in a slow transit constipation model in rats can increase the expression levels of glial cell-derived neurotrophic factor (GDNF) in colonic tissues, repair the ultramicrostructure of the colonic tissues, thereby improving the intestinal transit ability of the model rats, and effectively alleviating constipation symptoms (34). This evidence concerns the gene GDNF and constipation disorder.