Genome-wide screen by CRISPRa library in vivo for drivers of hepatocellular carcinoma (HCC) progression revealed that overexpression of XAGE1B, PLK4, LMO1 and MYADML2 genes promoted HCC cell proliferation and invasion, which were suppressed by their inhibition. The gene discussed is XAGE1B; the disease is hepatocellular carcinoma.